SNAP* CANINE PARVOVIRUS ANTIGEN TEST KIT
Canine Parvovirus Antigen Test Kit
For veterinary use only.
The SNAP Canine Parvovirus Antigen Test Kit is a rapid
enzyme immunoassay for the detection of canine parvovirus (CPV) antigen
in canine feces. This test detects a surface protein antigen of CPV
(including intact virus particles) shed in the feces of CPV-infected
Each kit contains five sampling swabs with
anti-parvovirus:HRPO conjugate and five SNAP devices. Each SNAP device
contains 0.4 mL of wash solution and 0.6 mL of substrate solution.
- Store at 2°-25°C until the expiration date.
- All components must be at room temperature (15°-30°C)
before running the test. Do not heat. This will take at least 30
minutes depending upon the temperature of your laboratory.
Precautions and Warnings
- Do not mix components from kits with different lot numbers.
- Properly dispose of contaminated materials and disinfect work areas.
- Do not use a SNAP device that has been activated prior to the addition of sample.
- The sampling swab is not designed to be used rectally.
- Lubricants may reduce test sensitivity by reducing the volume of feces tested.
- Samples must be at room temperature (15°-30°C) before beginning the test procedure.
- Canine fecal matter is required for this test. Swabs are provided for sampling.
- Fecal samples can be stored at 2°-8°C for 24 hours. If longer storage is required, samples should be frozen.
1. If stored in a refrigerator, allow all components to equilibrate at room temperature (15°-30°C) for 30 minutes. Do not heat.
2. Obtain a sampling swab and a
SNAP device for each sample to be tested. Pull and twist the tube
covering the swab tip to remove the tube from the swab/reagent bulb
assembly (A). Using the swab, coat the swab tip with fecal material.
Then, return the swab to the tube (B).
4. Place the SNAP device on a
horizontal surface. Using the swab as a pipette, dispense 5 drops of
the fluid into the sample well, being careful not to splash contents
outside of the sample well (Figure 1).
5. When color FIRST appears in the activation circle, push the activator firmly until it is flush with the device body (Figure 2).
6. Read the test result at 8 minutes.
Interpreting the Test Results
To determine the test result, read the reaction spots
in the result window and compare the color intensity of the sample spot
to that of the negative control spot.
Color development in the sample spots that is darker
than the negative control indicates a positive result, and the presence
of parvovirus antigen in the sample.
Color development only in the positive control spot indicates a negative result.
- Negative control (safeguard against false-positives)-If
color in the negative control spot is equal to or darker than the color
in the sample spot, the result is invalid and the sample should be
- No color development-If the positive control does not develop color, repeat the test.
- Background-If the sample is
allowed to flow past the activation circle, background color may
result. Some background color is normal. However, if colored background
obscures the test result, repeat the test.
Sensitivity and Specificity
Sample SizeKit/ Reference
Relative Sensitivity and Specificity95% Confidence Limit
Sen., 100% (95% CL 94%-100%)Spec., 100% (95% CL 89%-100%)
Sen., 100% (95% CL 98%-100%)Spec., 98% (95% CL 93%-99.8%)
Parvo Vaccine Cross-Reactivity-In a
study of 64 dogs vaccinated with six different modified live CPV-2
vaccines, the SNAP Parvovirus Antigen Test Kit did not detect CPV-2 in
A population of 64 beagles with low or no antibody to
canine parvovirus-2 (CPV-2) were vaccinated with one of five different
combination vaccines (Duramune® Max 5, Ft. Dodge Animal Health; Progard® 5, Intervet; Vanguard® Plus 5L, Pfizer Animal Health; Recombitek® C4, Merial; Galaxy® DA 2PPv, Schering-Plough Animal Health) or one monovalent product (NeoPar®
NEOTECH LLC) containing modified live CPV-2 vaccine. Fecal samples were
collected on day 0 and on one or more of the following days: 3, 4, 5, 6
and 7 post-vaccination. All samples were tested for CPV-2 using the
SNAP Parvovirus Antigen Test Kit. No cross-reactivity was detected.
IDEXX Technical Support
Europe 00800 1234 3399
U.S. Vet. License No. 313
Product Code 5024.03
*SNAP is a trademark or a registered trademark of IDEXX Laboratories, Inc. in the United States and/or other countries.
All other products and company names and logos are trademarks of their respective holders.
Covered by U.S. Patent Nos. 5,726,010; 5,726,013; 5,750,333; and 6,007,999. Other U.S. and/or foreign patents issued or pending.
© 2008 IDEXX Laboratories, Inc. All rights reserved.
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