The SNAP 3Dx Test Kit is an enzyme immunoassay for the simultaneous detection of Dirofilaria immitis antigen, antibody to Borrelia burgdorferi, and antibody to Ehrlichia canis in canine whole blood, serum or plasma.

Heartworm disease		Heartworm disease is caused by the filarial nematode D. immitis and has worldwide distribution. The insect vector for D. immitis is the mosquito. The detection of heartworm antigen is diagnostic for infection by D. immitis.
Canine ehrlichiosis		Ehrlichiosis tick-borne disease of dogs caused by the rickettsial parasite E. canis. Diagnosis of canine ehrlichiosis has been made by observation of typical clinical signs and by the measurement of a significant antibody titer to E. canis.1 A positive result on the SNAP® E. canis Antibody Test indicates a significant antibody titer to E. canis.
Lyme disease		The causitive agent of Lyme disease has been identified as the tick-borne spirochete Borrelia burgdorferi.2 An assessment of infection to B. burgdorferi can be made by measurement of canine antibody specific for B. burgdorferi.3 The SNAP® Lyme Antibody Test has been designed to demonstrate infection to B. burgdorferi.
Storage		SNAP devices and test reagents are stable until the expiration date when stored at 2°–7°C (36°–45°F). Room-temperature storage (optional): SNAP devices and reagents can be stored at room temperature (15°–27°C/59°–80°F) for 90 days or until the printed expiration date, whichever occurs first. After SNAP devices and reagents are removed from 2°–7°C (36°–45°F) storage for more than 24 hours, the expiration date is 90 days or the printed expiration date, whichever occurs first. If the 90-day expiration date occurs prior to the printed expiration date, record the new date in the space provided on the kit. All components must be at room temperature before running the test. This may take 30 minutes, depending on the temperature of your laboratory—do not heat.
Sample information		Samples must be at room temperature before beginning the test procedure. Serum or plasma, either fresh, previously frozen or stored at 2°–7°C (36°–45°F) can be used in this test. Serum or plasma can be stored for up to seven days at 2°–7°C (36°–45°F). For longer storage, the sample should be frozen (-20°C/-4°F or colder). Previously frozen or older samples must be centrifuged before use. Hemolyzed or lipemic samples will not affect results. EDTA or heparin in plasma will not affect results. Whole blood can be used. Whole blood must be anticoagulated (e.g., EDTA, heparin) and can be used either fresh or after refrigeration (2°–7°C/36°–45°F) for up to one week.
Sensitivity and Specificity