2. Holding bottle vertical, add 5 drops of conjugate to the sample tube (blue cap).

3. Cap the sample tube and mix thoroughly by inverting tube 3-5 times.

4. Place the device on a flat surface. Add contents of sample tube to Sample Well, being careful not to splash contents outside of Sample Well.
Sample will flow across Result Window, reaching Activate Circle in approximately 30-60 seconds. Some sample may remain in Sample Well.
Watch carefully for sample or blue color in the Activate Circle.

When Color First Appears In Activate Circle Push Activator Firmly Until It Is Flush With The Device Body.
note

Some samples may not flow to the Activate Circle within 60 seconds, and, therefore, the circle may not turn color. In this case, press the Activator after sample has flowed across Result Window.

Keep the device horizontal to ensure accurate results.

5. Read test result at

10 Minutes.
note

positive control may develop sooner, but results are not complete until 10 minutes.

Interpreting Test Result

To determine the test result, read the reaction spots in the Result Window. Color development in antigen indicator spots is proportional to the heartworm antigen concentration in the sample indicating low or high antigen levels. If no color develops in the positive control spot, repeat the test.

Negative Result

Only positive control spot develops color.

Positive Result

1. Low Antigen Level

Positive control and low antigen indicator develop color.

2. High Antigen Level

Positive control and both antigen indicators develop color.

Reaction With Negative Control

The negative control spot serves as a safeguard against false positives and helps indicate that the assay has been run properly.

1. Positive Result

If color in the high and/or low antigen indicator is darker than negative control spot, result is positive.

2. Invalid Result

If color in the negative control is equal to or darker than high and/or low antigen indicator, repeat the test.

Invalid Results

1. Background

If the sample is allowed to flow past the activate circle, background color may result. Some background color is normal. However, if colored background obscures test result, repeat the test.

2. No Color Development

If positive control does not develop color, repeat the test.

Sensitivity & Specificity Of Snap Feline Heartworm Antigen Test Kit using Characterized Positive And Negative Samples
study id	sample Size kit/reference				sample type	relative Sensitivity & Specificity 95% Confidence Limit	kappa statistic
	+/+	-/+	+/-	-/-
1	56	8	0	100	serum	sen., 87.5% (95% Cl 0.768 - 0.944) spec., 100% (95% Cl 0.974 - 1.0)	0.895

study Conducted At Idexx Laboratories. The 64 Positive Samples Were Characterized By Necropsy; The 100 Negative Samples Have Been Non-reactive On All Heartworm Antigen Tests.

* Snap Is Either A Trademark Or A Registered Trademark Of Idexx Laboratories, Inc. In The United States And/or Other Countries.

covered By U.s. Patent Nos. 5,627,026; 5,726,010; 5,726,013; 5,750,333; And/or 6,007,999. Other U.s. And/or Foreign Patents Issued Or Pending.

1. Rawlings, C.a., Et Al. 1982. Four Types Of Occult dirofilaria Immitis infection In Dogs. javma.180: Pp.1323-1326.

for Questions, Comments Or To Report Any Unusual Results, Call Idexx Customer Support

Usa/canada 800-248-2483

Europe 00 800 1234 3399

Australia 1800 655 978

If Possible, Please Have Your Kit Lot Number Available.

Manufacturer: IDEXX Laboratories, Inc. One IDEXX Drive, Westbrook, Maine 04092, USA

U.S. Vet License No. 313

Product Code 5F20.00

© 2002 IDEXX Laboratories, Inc.

06-04256-01