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A: TO TEST >400 mg/dl:
Drop the filled 5 microliter pipette(s) into the diluent vial (one pipette if serum is used or two pipettes if whole blood is used).
B: TO TEST >800 mg/dl:
Drop one 5 microliter pipette of whole blood or alternatively, 2.5 microliters of serum into the diluent vial.
1. Allow 5 minutes for the specimen to disperse throughout the diluent. Shake well to insure mixing before removing a sample for testing.
2. Use a plastic Pasteur pipette to draw a small amount of the specimen-diluent mixture, making sure that excess is removed from outside of the pipette. Deliver 2 drops inside the first slide ring, 3 drops inside the second ring and 4 drops inside the third ring of the glass slide. Take care to hold the pipette vertically to insure accurate dispensing of proper size drops.
3. Shake latex bottle to insure mixing. Remove cap and add 2 drops of latex to each slide ring. Hold dropper bottle vertically to insure accurate dispensing of proper size drops.
4. Use the provided mixing stick to stir the drops and spread them over the entire area inside the rings.
5. Gently tilt and rotate the slide to maintain suspension of the reactants.
6. Observe for agglutination after rotating for approximately 15 seconds. If agglutination does not occur in all 3 rings, continue to rotate for an additional 45 seconds. Read slide and record finding at this time.
Some samples that are truly negative will appear to have glossy type patches floating on top. This is not to be considered a positive agglutination. The glossy sheen and patches on top are almost always linked with a negative sample.
Interpretation: Timing is important. Specimens with high IgG levels will agglutinate in all three rings within 15 seconds. When agglutination has not occurred in all 3 rings by 15 seconds, the slide should be rotated an additional 45 seconds, then read. The following table should be used to interpret the results at this time.
all 3 rings+
- + +
- - +
- - -
Important: If one or more rings have a questionable or very weak agglutination reaction, these rings should be considered negative.
Precautions: The accuracy of this test is dependent upon delivery of consistent-size drops of both latex and serum diluents. It is, therefore, extremely important that care be taken to hold the dropper bottle and Pasteur pipette vertically while dispensing drops. Also, care must be taken to avoid delivering air bubbles with the drops of liquid. The agglutination slide should be rinsed with water and dried between tests.
Latex: If latex is difficult to squeeze from vial, or if it has a stringy appearance, DO NOT USE. Contact technical support.
Diluent: The diluent can become contaminated if opened prior to use. If diluent has a cloudy appearance, DO NOT USE.
STORAGE: Store latex between 35°F and 45°F. Latex can degrade at extremes of low and/or high temperatures.